Referenced in: none

Automatically associated channels: Kv10.1

Title: Primary epithelial cell models for cystic fibrosis research.

Authors: Scott H Randell, M Leslie Fulcher, Wanda O'Neal, John C Olsen

Journal, date & volume: Methods Mol. Biol., 2011 , 742, 285-310

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/21547740

Abstract
When primary human airway epithelial (hAE) cells are grown in vitro on porous supports at an air-liquid interface (ALI), they recapitulate in vivo morphology and key physiologic processes. These cultures are useful for studying respiratory tract biology and diseases and for testing new cystic fibrosis (CF) therapies. This chapter gives protocols enabling creation of well-differentiated primary CF and non-CF airway epithelial cell cultures with non-proprietary reagents. We also discuss the production of retroviral and lentiviral vectors, the derivation of hAE cell lines, reporter gene assays, and the evolving science of gene overexpression and knockdown in ALI hAE cultures.