Referenced in: none

Automatically associated channels: TRP , TRPM , TRPM7

Title: The Mg2+ transporter MagT1 partially rescues cell growth and Mg2+ uptake in cells lacking the channel-kinase TRPM7.

Authors: Francina Deason-Towne, Anne-Laure Perraud, Carsten Schmitz

Journal, date & volume: FEBS Lett., 2011 Jul 21 , 585, 2275-8

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/21627970

Abstract
Magnesium (Mg(2+)) transport across membranes plays an essential role in cellular growth and survival. TRPM7 is the unique fusion of a Mg(2+) permeable pore with an active cytosolic kinase domain, and is considered a master regulator of cellular Mg(2+) homeostasis. We previously found that the genetic deletion of TRPM7 in DT40 B cells results in Mg(2+) deficiency and severe growth impairment, which can be rescued by supplementation with excess extracellular Mg(2+). Here, we show that gene expression of the Mg(2+) selective transporter MagT1 is upregulated in TRPM7(-/-) cells. Furthermore, overexpression of MagT1 in TRPM7(-/-) cells augments their capacity to uptake Mg(2+), and improves their growth behavior in the absence of excess Mg(2+).