Referenced in: none

Automatically associated channels: Kv7.1

Title: Hypothyroidism of gene-targeted mice lacking Kcnq1.

Authors: Henning Fröhlich, Krishna M Boini, Guiscard Seebohm, Nathalie Strutz-Seebohm, Oana N Ureche, Michael Föller, Melanie Eichenmüller, Ekaterina Shumilina, Ganesh Pathare, Anurag Kumar Singh, Ursula Seidler, Karl E Pfeifer, Florian Lang

Journal, date & volume: Pflugers Arch., 2011 Jan , 461, 45-52

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/20978783

Abstract
Thyroid hormones T3/T4 participate in the fine tuning of development and performance. The formation of thyroid hormones requires the accumulation of I(-) by the electrogenic Na(+)/I(-) symporter, which depends on the electrochemical gradient across the cell membrane and thus on K(+) channel activity. The present paper explored whether Kcnq1, a widely expressed voltage-gated K(+) channel, participates in the regulation of thyroid function. To this end, Kcnq1 expression was determined by RT-PCR, confocal microscopy, and thyroid function analyzed in Kcnq1 deficient mice (Kcnq1 ( -/- )) and their wild-type littermates (Kcnq1 ( +/+ )). Moreover, Kcnq1 abundance and current were determined in the thyroid FRTL-5 cell line. Furthermore, mRNA encoding KCNQ1 and the subunits KCNE1-5 were discovered in human thyroid tissue. According to patch-clamp TSH (10 mUnits/ml) induced a voltage-gated K(+) current in FRTL-5 cells, which was inhibited by the Kcnq inhibitor chromanol (10 μM). Despite a tendency of TSH plasma concentrations to be higher in Kcnq1 ( -/- ) than in Kcnq1 ( +/+ ) mice, the T3 and T4 plasma concentrations were significantly smaller in Kcnq1 ( -/- ) than in Kcnq1 ( +/+ ) mice. Moreover, body temperature was significantly lower in Kcnq1 ( -/- ) than in Kcnq1 ( +/+ ) mice. In conclusion, Kcnq1 is required for proper function of thyroid glands.