Referenced in: none

Automatically associated channels: ClC4 , Kv10.1 , Slo1

Title: Clcn4-2 genomic structure differs between the X locus in Mus spretus and the autosomal locus in Mus musculus: AT motif enrichment on the X.

Authors: Di Kim Nguyen, Fan Yang, Rajinder Kaul, Can Alkan, Anthony Antonellis, Karen F Friery, Baoli Zhu, Pieter J de Jong, Christine M Disteche

Journal, date & volume: Genome Res., 2011 Mar , 21, 402-9

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/21282478

Abstract
In Mus spretus, the chloride channel 4 gene Clcn4-2 is X-linked and dosage compensated by X up-regulation and X inactivation, while in the closely related mouse species Mus musculus, Clcn4-2 has been translocated to chromosome 7. We sequenced Clcn4-2 in M. spretus and identified the breakpoints of the evolutionary translocation in the Mus lineage. Genetic and epigenetic differences were observed between the 5'ends of the autosomal and X-linked loci. Remarkably, Clcn4-2 introns have been truncated on chromosome 7 in M. musculus as compared with the X-linked loci from seven other eutherian mammals. Intron sequences specifically preserved in the X-linked loci were significantly enriched in AT-rich oligomers. Genome-wide analyses showed an overall enrichment in AT motifs unique to the eutherian X (except for genes that escape X inactivation), suggesting a role for these motifs in regulation of the X chromosome.