PubMed 21330840

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Automatically associated channels: ClIC1

Title: CLIC1 protein: a candidate prognostic biomarker for malignant-transformed hydatidiform moles.

Authors: Zhong-Hua Shi, Chun Zhao, Hong Wu, Wei Wang, Xiao-Mei Liu

Journal, date & volume: Int. J. Gynecol. Cancer, 2011 Jan , 21, 153-60

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The purpose of this study was to identify prognostic biomarkers indicating malignant transformation of hydatidiform moles (HMs).Two-dimensional gel electrophoresis-based proteomic approach was used to compare the protein profiles of complete benign moles (3 samples) with those of malignant-transformed moles (3 samples). Matrix-assisted laser desorption/ionization time of flight mass spectrometry was used to identify differentially expressed proteins. Western blot was used to verify the results of 2-dimensional gel electrophoresis, and immunohistology was used to explore the function of these proteins in gestational trophoblastic disease.Eighteen proteins, deregulated in the malignant-transformed group compared with the benign group (ratio ≥ 2; P < 0.05), were identified. A bioinformatic analysis indicated that most of these 18 proteins were involved in the processes of cell proliferation and cell survival. Among the 18 proteins, chloride intracellular channel protein 1 (CLIC1) was chosen for further study. Our results showed that the levels of CLIC1 expression in choriocarcinoma tissue were higher than in complete HM tissue (P < 0.01). Chloride intracellular channel protein 1 expression was increased in the tissues of malignant-transformed HMs compared with nontransformed HMs (P < 0.01).Our findings suggest that CLIC1 could be a potential new prognostic biomarker for hydatidiform mole that undergoes malignant transformation.