Referenced in: none

Automatically associated channels: Kv1.2 , Kv2.1 , Kv3.1 , Slo1

Title: Contribution of the delayed-rectifier potassium channel Kv2.1 to acute spinal cord injury in rats.

Authors: Min-Young Song, Youn Joo Moon, Seok Kyo Shin, Tae Yong Kim, Tae Young Yune, Kang-Sik Park

Journal, date & volume: BMB Rep, 2010 Nov , 43, 756-60

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/21110920

Abstract
Recent studies have reported that delayed-rectifier Kv channels regulate apoptosis in the nervous system. Herein, we investigated changes in the expression of the delayed-rectifier Kv channels Kv1.2, Kv2.1, and Kv3.1 after acute spinal cord injury (SCI) in rats. We performed RT-PCR analysis and found an increase in the level of Kv2.1 mRNA after SCI but no significant changes in the levels of Kv1.2 and Kv3.1 mRNA. Western blot analysis revealed that Kv2.1 protein levels rapidly decreased and then dramatically increased from 1 day, whereas Kv3.1b protein levels gradually and sharply decreased at 5 days. Kv1.2 protein levels did not change significantly. In addition, Kv2.1 clusters were disrupted in the plasma membranes of motor neurons after SCI. Interestingly, the expressional changes and translocation of Kv2.1 were consistent with the apoptotic changes on day 1. Therefore, these results suggest that Kv2.1 channels probably contribute to neuronal cell responses to SCI.