Referenced in: none

Automatically associated channels: Kir4.1 , Kv7.1 , Slo1

Title: Kir4.1 channel expression is essential for parietal cell control of acid secretion.

Authors: Penghong Song, Stephanie Groos, Brigitte Riederer, Zhe Feng, Anja Krabbenhöft, Michael P Manns, Adam Smolka, Susan J Hagen, Clemens Neusch, Ursula Seidler

Journal, date & volume: , 2011 Mar 2 , ,

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/21367857

Abstract
Kir4.1 channels were found to colocalize with the H(+)/K(+)-ATPase throughout the parietal cell (PC) acid secretory cycle. This study was undertaken to explore their functional role. Acid secretory rates, electrophysiological parameters, PC ultrastructure, and gene and protein expression were determined in gastric mucosae of 7-8-day-old Kir4.1-deficient mice and WT littermates. Kir4.1(-/-) mucosa secreted significantly more acid and initiated secretion significantly faster than WT mucosa. No change in PC number but a relative up-regulation of H(+)/K(+)-ATPase gene and protein expression (but not of other PC ion transporters) was observed. Electron microscopy revealed fully fused canalicular membranes and a lack of tubulovesicles in resting state Kir4.1(-/-) PCs, suggesting that Kir4.1 ablation may also interfere with tubulovesicle endocytosis. The role of this inward rectifier in the PC apical membrane may therefore be to balance between K(+) loss via KCNQ1/KCNE2 and K(+) reabsorption by the slow turnover of the H(+)/K(+)-ATPase, with consequences for K(+) reabsorption, inhibition of acid secretion, and membrane recycling. Our results demonstrate that Kir4.1 channels are involved in the control of acid secretion and suggest that they may also affect secretory membrane recycling.