Referenced in: none

Automatically associated channels: Kir1.1 , Kir6.1

Title: Kir1.1 expression in embryonic kidney epithelia.

Authors: Gerald S Braun, Michael F Horster, Stephan M Huber

Journal, date & volume: Biochem. Biophys. Res. Commun., 2003 Dec 26 , 312, 1191-5

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/14651999

Abstract
K(+) channels may regulate cell cycling, cell volume, and cell proliferation. We have recently shown a role for an inwardly rectifying K(+) channel, Kir6.1/SUR2(B), in the regulation of cell proliferation during early kidney development. Here, we show that the protein of a further K(+) channel, Kir1.1 (ROMK), is also developmentally expressed in prenatal rat kidney epithelia. In the embryonic stage, Kir1.1 protein was localized to the plasma membrane of ureteric buds and collecting ducts, and of nephron stages up to the comma-shaped body. Experimental increase in cAMP upregulated Kir1.1b (ROMK2) mRNA abundance in ureteric buds. Kir1.1 protein was restricted to the distal nephron during later postnatal development and adulthood, as has been reported. In conclusion, we demonstrate redundancy of Kir channel expression in early embryonic kidney which could suggest that Kir1.1 acts in a similar way as Kir6.1/SUR2(B) to promote cell proliferation or other developmental functions.