Referenced in: none

Automatically associated channels: Kir2.3

Title: Effect of axotomy on the cyclic GMP increase induced by preganglionic stimulation and high extracellular K+ concentration in superior cervical sympathetic ganglion of the rat.

Authors: M Ando, T Nanba, M Okuya, Y Nagata

Journal, date & volume: Brain Res., 1983 May 16 , 267, 313-21

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/6307468

Abstract
Cyclic GMP generation, induced by preganglionic nerve stimulation or by high extracellular potassium ion concentration (70 mM) in the medium, was studied during aerobic incubation of the excised superior cervical sympathetic ganglion of the rat with and without axotomy, and the results were compared with that of preganglionic denervation. Both axotomy and denervation of the ganglion for a week caused complete loss of increase of cyclic GMP content in the ganglion in response to the preganglionic nerve stimulation. However, the increase of the ganglionic cyclic GMP content evoked by raising the extracellular potassium ion concentration was maintained at a level about two-thirds of the control after axotomy for 1-4 days, while it was abolished within a day after denervation. Ganglionic choline acetyltransferase activity was maintained for several days after axotomy, but it was decreased rapidly by denervation. Acetylcholinesterase, dopamine-beta-hydroxylase and muscarinic cholinergic receptor sites had been lost almost completely a week after axotomy. These results suggest that the increase of ganglionic cyclic GMP content caused by depolarization with high extracellular K+ is associated with the preganglionic nerve terminals rather than with postganglionic receptor.