Referenced in: none

Automatically associated channels: Kir6.2

Title: Differential expression of GABAA receptor alpha-subunits in rat brain during development.

Authors: R M McKernan, P Cox, N P Gillard, P Whiting

Journal, date & volume: FEBS Lett., 1991 Jul 29 , 286, 44-6

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/1650715

Abstract
Unique cytoplasmic loop regions of the alpha 1, alpha 2, alpha 3, and alpha 5 subunits of the GABAA receptor have been expressed in E. coli and used to generate polyclonal antisera specific for these subunits. The antibodies identify proteins by SDS-polyacrylamide gel electrophoresis and western blotting of molecular size 51 kDa, 53 kDa, 59 kDa and 55 kDa, respectively, which show differential patterns of expression during development. Whereas the alpha 2 and alpha 3 subunits are present at early stages, the expression of alpha 1 and alpha 3 subunits is low at birth and increases with age. This differential expression could be correlated with previous studies examining the developmental expression of BZ1 and BZ2 benzodiazepine binding sites.