Referenced in: none

Automatically associated channels: Cav1.2

Title: Identification of a cardiac isoform of the murine calcium channel alpha1C (Cav1.2-a) subunit and its preferential binding with the beta2 subunit.

Authors: Manabu Murakami, Takayoshi Ohba, Yoichiro Takahashi, Hiroyuki Watanabe, Ichiro Miyoshi, Shinsuke Nakayama, Kyoichi Ono, Hiroshi Ito, Toshihiko Iijima

Journal, date & volume: J. Mol. Cell. Cardiol., 2006 Jul , 41, 115-25

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/16787652

Abstract
We describe a cardiac muscle isoform of the voltage-dependent calcium channel alpha1 subunit, which corresponds to the rabbit ortholog of alpha1C-a (Cav1.2a). We also cloned smooth muscle isoforms alpha1C-b (Cav1.2b) and alpha1C-d (Cav1.2d). Differences among these three isoforms lie within the N-terminal region (exon 1A or 1B), the sixth transmembrane segment of domain I (exon 8A or 8B), and the use of exon 10, which forms the intracellular loop between transmembrane domains I and II. Two-hybrid analysis revealed interactions among the three alpha1 isoforms and beta subunits. In vitro overlay and immunoprecipitation analyses revealed preferential binding between alpha1C-a and beta2, which is also expressed at a high level in the heart.