Referenced in: none

Automatically associated channels: Kv11.1 , Kv7.1 , Nav1.5

Title: Denaturing high-performance liquid chromatography screening of the long QT syndrome-related cardiac sodium and potassium channel genes and identification of novel mutations and single nucleotide polymorphisms.

Authors: Ling-Ping Lai, Yi-Ning Su, Fon-Jou Hsieh, Fu-Tien Chiang, Jyh-Ming Juang, Yen-Bin Liu, Yi-Lwun Ho, Wen-Jone Chen, San-Jou Yeh, Chun-Chieh Wang, Yu-Lin Ko, Tsu-Juey Wu, Kwo-Chang Ueng, Meng-Huan Lei, Hsuan-Ming Tsao, Shih-Ann Chen, Tin-Kwang Lin, Mei-Hwan Wu, Huey-Ming Lo, Shoei K Stephen Huang, Jiunn-Lee Lin

Journal, date & volume: J. Hum. Genet., 2005 , 50, 490-6

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/16155735

Abstract
Mutations in cardiac potassium and sodium channel genes are responsible for several hereditary cardiac arrhythmia syndromes. We established a denaturing high-performance liquid chromatography (DHPLC) protocol for rapid mutation screening of these genes, and reported mutations and variations identified by this method. We included 28 patients with Brugada syndrome, 4 with congenital long QT syndrome (LQTS), 11 with drug-induced LQTS, 4 with idiopathic ventricular fibrillation, and 50 normal volunteers. Polymerase chain reactions were performed to amplify the entire coding region of these genes. DHPLC was used to screen for heteroduplexes then DNA sequencing was performed. With this method, we identified the mutation(s) in all four patients with congenital LQTS (KCNQ1 A341V, KCNH2 N633D, KCNH2 2768Cdel and KCNE1 K70 N Y81C double mutations). We also identified the SCN5A A551T mutation in 1 of the 28 patients with Brugada syndrome. All the above-mentioned mutations were novel except KCNQ1 A341V. No mutations were identified in patients with drug-induced LQTS or idiopathic ventricular fibrillation. In total, 25 single nucleotide polymorphisms were identified, 10 of which were novel. In conclusion, DHPLC is a sensitive and rapid method for detection of cardiac sodium and potassium channel gene mutations.