Referenced in: none

Automatically associated channels: ClC3 , ClC4

Title: Single-channel properties of volume-sensitive Cl- channel in ClC-3-deficient cardiomyocytes.

Authors: Jun Wang, Hongtao Xu, Shigeru Morishima, Shigeru Tanabe, Kouichi Jishage, Shinichi Uchida, Sei Sasaki, Yasunobu Okada, Takahiro Shimizu

Journal, date & volume: Jpn. J. Physiol., 2005 Dec , 55, 379-83

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/16441975

Abstract
It is controversial whether the ClC-3 protein, which is one of the voltage-dependent chloride channel ClC family members, is a candidate for the volume-sensitive outwardly rectifying (VSOR) Cl(-) channel per se or its regulator. Here, for the first time, we examined the single-channel properties of the VSOR Cl(-) channel in ventricular myocytes isolated from ClC-3-deficient mice. The single-channel current induced by cell swelling exhibited Cl(-) selectivity, mild outward rectification, and an intermediate unitary conductance (around 38 pS). A Cl(-) channel blocker, 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS), reversibly inhibited the outward current. These single-channel properties were identical with those in ClC-3 expressing wild-type ventricular myocytes. These results indicate that the single-channel activity of the VSOR Cl(-) channel is independent of the expression of ClC-3 proteins in mouse ventricular myocytes.