Referenced in: none

Automatically associated channels: Nav1.2 , Slo1

Title: G-protein activation modulates pseudo-periodic oscillation of Na channel.

Authors: Sriparna Majumdar, Sujit K Sikdar

Journal, date & volume: Biochem. Biophys. Res. Commun., 2006 Aug 25 , 347, 444-51

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/16831405

Abstract
We have shown before that the duration and amplitude of both prolonged (1-160 s) and short (100-1000 ms) depolarizing prepulse altered all the steady-state and kinetic parameters of rNav1.2a voltage-gated sodium channel in a pseudo-oscillatory fashion with variable time period and amplitude, often superimposed on a linear trend. In this study, we have examined the effect of G-protein activation on pseudo-oscillatory properties of the rNav1.2a sodium channel alpha subunit, heterologously expressed in Chinese hamster ovary cells. G-protein modification caused insignificant changes in the slow pseudo-periodic oscillation of the activation properties of sodium channel; only the time period of the oscillation was altered from approximately 30 to 21s. In contrast, G-protein activation abolished the faster component of pseudo-periodic oscillation in steady-state inactivation properties of sodium channel; the conditioning duration dependence of steady-state inactivation becomes monotonic in nature.