Referenced in: none

Automatically associated channels: ClC3 , ClC4

Title: Cholesterol is critical to the integrity of neuronal porosome/fusion pore.

Authors: Aleksandar Jeremic, Won Jin Cho, Bhanu P Jena

Journal, date & volume: , 2006 Jun-Jul , 106, 674-7

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/16709444

Abstract
Secretion is one of the most fundamental cellular processes. Porosomes have been demonstrated as the universal secretory machinery in cells. Earlier studies determine the presence of a number of proteins in porosomes, among them the N- and P/Q-type calcium channels, actin, syntaxin-1, synaptotagmin-1, vimentin, the N-ethylmaleimide-sensitive factor (NSF), the chloride channel CLC-3, and the alpha subunit of the heterotrimeric GTP-binding protein G(o). Studies demonstrate that t-SNAREs localize at the base of porosomes, and directly interact with calcium channels. In the present study, we demonstrate that Syntaxin-1 co-localizes with cholesterol in solubilized synaptosomal membrane preparations. Depletion of cholesterol, results in the dissociation of both Syntaxin-1 and N-type calcium channel from neuronal porosomes. Thus, cholesterol participates as an integral component of the neuronal porosome complex, and is required for its stability.