Channelpedia

PubMed 14964862


Referenced in: none

Automatically associated channels: Kv10.1



Title: Production of antibodies to alpha(181-192) peptides of neuronal nicotinic acetylcholine receptor coupled to protein carriers in different orientations.

Authors: M V Skok, O Yu Lykhmus, S O Bobrovnik, O V Solodova, S J Tzartos, T Tsouloufis, R Vanderesse, M Marraud

Journal, date & volume: Ukr. Biokhim. Zh., 2002 Jul-Aug , 74, 54-60

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/14964862


Abstract
The antibodies to nicotinic acetylcholine receptor alpha(181-192) synthetic peptides were elicited in rabbits and mice using the peptides conjugated to protein carriers in different orientations, either through C-terminal Cys (S-conjugates), or through amino groups (N-conjugates). S-conjugated peptides were less potent in eliciting peptide-specific antibodies compared to N-conjugates and this type of conjugation resulted in antibodies to the coupling reagent. However, the epitopes present in either S- or N-conjugated peptides appeared to be similar, indicating that amino acid residues, which form the epitope, were located in the middle part of the peptide and did not include both N- and C-terminal residues. Peptide conjugation to a protein carrier did not play a role in stabilizing the peptide conformation, but was necessary to concentrate the peptide epitopes on the carrier surface enabling bivalent antibody binding.