Referenced in: none

Automatically associated channels: ClC3 , ClC4

Title: Hypertonic saline solution stimulated CLC-3 production in primary cultured human nasal epithelial cells.

Authors: Hua-Bin Li, Jian-Bo Shi, Fang Cao, Lei Cheng, Wei-Ping Wen, Geng Xu

Journal, date & volume: , 2008 Mar , 35, 47-51

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/17869465

Abstract
To determine the molecular mechanisms of hypertonic saline on nasal disorders.Primary human nasal epithelial cells (HNECs) were treated with 0.9 and 3% saline for 12h. CLC-3 was detected in HNECs by immunohistochemistry, reverse transcriptase-polymerase chain reaction (RT-PCR) and western blotting.Positive expression of CLC-3 was detected in HNECs in all subjects by immunohistochemistry. The relative density of CLC-3 mRNA was 0.22+/-0.09 and 0.12+/-0.05 in HNECs treated with 3 and 0.9% saline, respectively. The effect of 3% saline on the expression of CLC-3 in HNECs was observed in a time-dependent manner.Hypertonic saline decreases the nasal edema and influences the mucocilliary function through the fluid secretion in vitro and may be associated with the upregulation of CLC-3.