Referenced in: none

Automatically associated channels: Kv10.1

Title: Potassium currents in primary cultured astrocytes from the rat corpus callosum.

Authors: Daniel Reyes-Haro, Ricardo Miledi, Jesús García-Colunga

Journal, date & volume: J. Neurocytol., 2005 Dec , 34, 411-20

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/16902762

Abstract
The corpus callosum (CC) is the main white matter tract in the brain and is involved in interhemispheric communication. Using the whole-cell voltage-clamp technique, a study was made of K(+)-currents in primary cultured astrocytes from the CC of newborn rats. These cells were positive to glial fibrillary acidic protein after culturing in Dulbecco's Modified Eagle Medium (> 95% of cells) or in serum-free neurobasal medium with G5 supplement (> 99% of cells). Astrocytes cultured in either medium displayed similar voltage-activated ion currents. In 81% of astrocytes, the current had a transient component and a sustained component, which were blocked by 4-aminopyridine and tetraethylammonium, respectively; and both had a reversal potential of -66 mV, indicating that they were carried by K(+) ions. Based on the Ba(2+)-sensitivity and activation kinetics of the K(+)-current, two groups of astrocytes were discerned. One group (55% of cells) displayed a strong Ba(2+) blockade of the K(+)-current whose activation kinetics, time course of decay, and the current-voltage relationship were modified by Ba(2+). This current was greatly blocked (52%) by Ba(2+) in a voltage-dependent way. Another group (45% of cells) presented weak Ba(2+)-blockade, which was only blocked 24% by Ba(2+). The activation kinetics and time course of decay of this current component were unaffected by Ba(2+). These results may help to understand better the roles of voltage-activated K(+)-currents in astrocytes from the rat CC in particular and glial cells in general.