Channelpedia

PubMed 17292857


Referenced in Channelpedia wiki pages of: none

Automatically associated channels: Kv10.1 , Kv2.1



Title: Interactions between alpha-conotoxin MI and the Torpedo marmorata receptor alpha-delta interface.

Authors: Leonardo Cortez, Cristina Marino-Buslje, Mirtha Biscoglio de Jiménez Bonino, Ulf Hellman

Journal, date & volume: Biochem. Biophys. Res. Commun., 2007 Mar 30 , 355, 275-9

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/17292857


Abstract
The muscle-type nicotinic receptor has two distinguishable acetylcholine binding sites at the alpha-gamma and alpha-delta subunit interfaces; alpha-conotoxins can bind them selectively. Moreover, we previously reported that alpha-conotoxin MI can interact with Torpedo californica and Torpedo marmorata receptors showing that conotoxins can also detect receptors from different species of the same genus [L. Cortez, S.G. del Canto, F. Testai, M.B. de Jiménez Bonino, Conotoxin MI inhibits the acetylcholine binding site of the Torpedo marmorata receptor, Biochem. Biophys. Res. Commun. 295 (2002) 791-795]. Herein, to identify T. marmorata receptor regions involved in alpha-conotoxin MI binding, a photoactivatable reagent was used and labeled sites were mapped by enzymatic proteolysis, MALDI-TOF-MS and Edman degradation. alpha-Conotoxin MI binding determinants were found and studies revealed a second binding motif at the alpha/delta interface. A proposal for receptor-toxin interaction is discussed based on experimental results and docking studies.