PubMed 18839021

Referenced in Channelpedia wiki pages of: none

Automatically associated channels: Nav1.3

Title: Deglycosylation altered the gating properties of rNav1.3: glycosylation/deglycosylation homeostasis probably complicates the functional regulation of voltage-gated sodium channel.

Authors: Qing Xu, Hui-Wen Cheng, Hui-Qiong He, Zhi-Rui Liu, Ming He, Hong-Tian Yang, Zhi-Lei Zhou, Yong-Hua Ji

Journal, date & volume: , 2008 Oct , 24, 283-7

PubMed link:

To examine the effect of deglycosylation on gating properties of rNav1.3.rNav1.3 was expressed in Xenopus oocyte, with glycosylation inhibition by using tunicamycin. Two-electrode voltage clamp was employed to record the whole-cell sodium current and data were analyzed by Origin software. Those of glycosylated rNav1.3 were kept as control.Compared with glycosylated ones, the steady-state activation curve of deglycosylated rNav1.3 was positively shifted by about 10 mV, while inactivation curve was negatively shifted by about 8 mV.Glycosylation altered the gating properties of rNav1.3 and contributed to the functional diversity.