Referenced in: none

Automatically associated channels: Cav1.2 , Cav1.3

Title: Direct interaction and functional coupling between voltage-gated CaV1.3 Ca2+ channel and GABAB receptor subunit 2.

Authors: Hye-Won Park, Hana Jung, Kee-Hyun Choi, Ja-Hyun Baik, Hyewhon Rhim

Journal, date & volume: FEBS Lett., 2010 Aug 4 , 584, 3317-22

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/20627102

Abstract
Although CaV1.2 and CaV1.3 are two subtypes of L-type Ca2+ channels expressed in the CNS, functions of CaV1.3 have not been well elucidated compared to CaV1.2. Here, we found that CaV1.3-NT associates with GABABR2-CT using yeast two-hybrid, GST pull-down and co-immunoprecipitation assays. We also demonstrated co-localization of CaV1.3 and GABABR2 in HEK293 cells and cultured hippocampal neurons. Whole-cell patch-clamp and Ca2+-imaging experiments revealed that activation of GABABR increases CaV1.3 currents and intracellular Ca2+ via CaV1.3, but not CaV1.2. These results show a physical and functional interaction between CaV1.3 and GABABR, suggesting the potential pivotal roles of CaV1.3 in the CNS.