Channelpedia

PubMed 20600762


Referenced in Channelpedia wiki pages of: none

Automatically associated channels: Kv1.5 , Kv11.1



Title: Aconitine blocks HERG and Kv1.5 potassium channels.

Authors: Yifu Li, Danna Tu, Hua Xiao, Yimei Du, Anruo Zou, Yuhua Liao, Shaohong Dong

Journal, date & volume: , 2010 Aug 19 , 131, 187-195

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/20600762


Abstract
Aconitum has been widely used to treat various diseases in China for a long time. However, improper use of this drug results in severe intoxication. Aconitine (ACO), a diterpenoid alkaloid from aconitum, mainly contributes to cardio-toxic effects of aconitum and has also been commonly known to induce arrhythmias in animal models. However, its pro-arrhythmic mechanisms are not clear.The effects of ACO on HERG and Kv1.5 channels were investigated.HERG and Kv1.5 channels were expressed in Xenopus laevis oocytes, and the resulting currents were recorded using a two-microelectrode voltage clamp technique.In HERG channels, ACO exhibited a blockade in a voltage- and time-dependent manner. The blockade was enhanced by further activation of currents, which were consistent with an open-channel blockade. In Kv1.5 channels, ACO produced a voltage-, time-, and frequency-dependent inhibition. The blockade was enhanced by higher rates of stimulation, consistent with preferential binding of the drug to the open state. In addition, ACO blocked Kv1.5 and HERG channels in a concentration-dependent manner with an IC(50) of 0.796+/-0.123 and 1.801+/-0.332 microM, respectively.ACO blocks HERG and Kv1.5 potassium channels in the open state. Blockade of potassium channels, particular the HERG channel, may be one of the important mechanisms of how ACO induces arrhythmias.