Referenced in: none

Automatically associated channels: ClC4 , ClC5

Title: ClC-5 regulates dentin development through TGF-beta1 pathway.

Authors: Xiaohong Duan, Yong Mao, Ting Yang, Xuan Wen, Huan Wang, Jin Hou, Yang Xue, Rong Zhang

Journal, date & volume: Arch. Oral Biol., 2009 Dec , 54, 1118-24

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/19878925

Abstract
ClC-5 is one of the voltage-dependent chloride channel (ClC) family members. Mutations involving CLCN5 cause an X-linked nephropathy associated with Dent's disease. Some Clcn5 gene knockout (ClC-5 KO) mice have abnormal growth of the teeth; however, the expression and function of ClC-5 during tooth development is still unknown. Herein we report abnormal dentin structure, decreased DSPP and increased TGF-beta1 protein level in ClC-5 KO teeth. In odontoblast-like MDPC-23 cells, the mRNA levels of Tgfb1, Dspp and Dmp-1 were upregulated with Clcn5 RNAi after 48h treatment; whilst there was no change in those of TGF-beta receptor Tgfbr1 and Tgfbr2. We suggest that the dentin changes in ClC-5 KO mice might be a result of increasing TGF-beta1, and the interplay between ClC-5 and TGF-beta1 needs further identified.