Referenced in: none

Automatically associated channels: Kv2.1

Title: Regulation of voltage-gated K+ channels by glucose metabolism in pancreatic beta-cells.

Authors: Masashi Yoshida, Katsuya Dezaki, Shiho Yamato, Atsushi Aoki, Hitoshi Sugawara, Hideo Toyoshima, San-E Ishikawa, Masanobu Kawakami, Masanori Nakata, Toshihiko Yada, Masafumi Kakei

Journal, date & volume: FEBS Lett., 2009 Jul 7 , 583, 2225-30

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/19500583

Abstract
Regulation of delayed rectifier-type K(+) channels (Kv-channels) by glucose was studied in rat pancreatic beta-cells. The Kv-channel current was increased in amplitudes by increasing glucose concentration from 2.8 to 16.6mM, while it was decreased by 2.8mM glucose in a reversible manner (down-regulation) in both perforated and conventional whole-cell modes. The current was decreased by FCCP, intrapipette 0mM ATP or AMPPNP. Glyceraldehyde, pyruvic acid, 2-ketoisocaproic acid, and 10mM MgATP prevented the down-regulation induced by 2.8mM or less glucose. The residual current after treatment with Kv2.1-specific blocker, guangxitoxin-1E, was unchanged by lowering or increasing glucose concentration. We conclude that glucose metabolism regulates Kv2.1 channels in rats beta-cells via altering MgATP levels.