Channelpedia

PubMed 18053989


Referenced in Channelpedia wiki pages of: none

Automatically associated channels: Kv1.1 , Kv1.2 , Kv1.3 , Kv1.4 , Kv1.6



Title: Expression and localization of Kv1 potassium channels in rat dorsal and ventral spinal roots.

Authors: Iku Utsunomiya, Eikichi Yoshihashi, Shinya Tanabe, Yoshihiko Nakatani, Hideaki Ikejima, Tadashi Miyatake, Keiko Hoshi, Kyoji Taguchi

Journal, date & volume: Exp. Neurol., 2008 Mar , 210, 51-8

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/18053989


Abstract
We investigated the expression and localization of Kv1 channels in dorsal spinal roots (DRs) and ventral spinal roots (VRs) in rats. Among Kv1.1-1.6 tested by RT-PCR, mRNAs of Kv1.1, 1.2, and 1.5 were moderately expressed, those of Kv1.3 and Kv1.6 were weakly expressed, and that of Kv1.4 was hardly expressed at all in both DRs and VRs, whereas all six mRNAs were detected in spinal cord. Western blotting revealed that the major immunoreactive proteins were Kv1.1 and Kv1.2 in both DRs and VRs. Quantitative analysis indicated that levels of Kv1.1 and Kv1.2 protein were significantly higher in DRs than VRs. Immunohistochemical examination showed that Kv1.1 and Kv1.2 were colocalized in juxtaparanodal regions of axons in both DRs and VRs. Finally, immunoprecipitation experiments revealed that Kv1.1 and Kv1.2 were coassembled. These findings indicate that Kv1 subtypes in DRs and VRs are somewhat different from those in spinal cord, and that the numbers of Kv1.1 and Kv1.2 channels are higher in DRs than VRs.