Channelpedia

Primary structure of the gamma subunit of the DHP-sensitive calcium channel from skeletal muscle.


Authors: S D Jay, S B Ellis, A F McCue, M E Williams, T S Vedvick, M M Harpold, K P Campbell

Journal, date & volume: Science, 1990 Apr 27 , 248, 490-2

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/2158672

Channelpedia reference in: cacng1

Abstract
Affinity-purified, polyclonal antibodies to the gamma subunit of the dihydropyridine (DHP)-sensitive, voltage-dependent calcium channel have been used to isolate complementary DNAs to the rabbit skeletal muscle protein from an expression library. The deduced primary structure indicates that the gamma subunit is a 25,058-dalton protein that contains four transmembrane domains and two N-linked glycosylation sites, consistent with biochemical analyses showing that the gamma subunit is a glycosylated hydrophobic protein. Nucleic acid hybridization studies indicate that there is a 1200-nucleotide transcript in skeletal muscle but not in brain or heart. The gamma subunit may play a role in assembly, modulation, or the structure of the skeletal muscle calcium channel.