Channelpedia

KCR1, a membrane protein that facilitates functional expression of non-inactivating K+ currents associates with rat EAG voltage-dependent K+ channels.


Authors: N Hoshi, H Takahashi, M Shahidullah, S Yokoyama, H Higashida

Journal, date & volume: J. Biol. Chem., 1998 Sep 4 , 273, 23080-5

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/9722534

Channelpedia reference in: Kv10.1

Abstract
Cerebellar granule neurons possess a non-inactivating K+ current, which controls resting membrane potentials and modulates the firing rate by means of muscarinic agonists. kcr1 was cloned from the cerebellar cDNA library by suppression cloning. KCR1 is a novel protein with 12 putative transmembrane domains and enhances the functional expression of the cerebellar non-inactivating K+ current in Xenopus oocytes. KCR1 also accelerates the activation of rat EAG K+ channels expressed in Xenopus oocytes or in COS-7 cells. Far-Western blotting revealed that KCR1 and EAG proteins interacted with each other by means of their C-terminal regions. These results suggest that KCR1 is the regulatory component of non-inactivating K+ channels.