Channelpedia

Molecular cloning and characterization of Kv6.3, a novel modulatory subunit for voltage-gated K(+) channel Kv2.1.


Authors: Yorikata Sano, Shinobu Mochizuki, Akira Miyake, Chika Kitada, Kohei Inamura, Hiromichi Yokoi, Katsura Nozawa, Hitoshi Matsushime, Kiyoshi Furuichi

Journal, date & volume: FEBS Lett., 2002 Feb 13 , 512, 230-4

PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/11852086

Channelpedia reference in: Kv6.3

Abstract
We report identification and characterization of Kv6.3, a novel member of the voltage-gated K(+) channel. Reverse transcriptase-polymerase chain reaction analysis indicated that Kv6.3 was highly expressed in the brain. Electrophysiological studies indicated that homomultimeric Kv6.3 did not yield a functional voltage-gated ion channel. When Kv6.3 and Kv2.1 were co-expressed, the heteromultimeric channels displayed the decreased rate of deactivation compared to the homomultimeric Kv2.1 channels. Immunoprecipitation studies indicated that Kv6.3 bound with Kv2.1 in co-transfected cells. These results indicate that Kv6.3 is a novel member of the voltage-gated K(+) channel which functions as a modulatory subunit.