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Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.

Robert L Strausberg, Elise A Feingold, Lynette H Grouse, Jeffery G Derge, Richard D Klausner, Francis S Collins, Lukas Wagner, Carolyn M Shenmen, Gregory D Schuler, Stephen F Altschul, Barry Zeeberg, Kenneth H Buetow, Carl F Schaefer, Narayan K Bhat, Ralph F Hopkins, Heather Jordan, Troy Moore, Steve I Max, Jun Wang, Florence Hsieh, Luda Diatchenko, Kate Marusina, Andrew A Farmer, Gerald M Rubin, Ling Hong, Mark Stapleton, M Bento Soares, Maria F Bonaldo, Tom L Casavant, Todd E Scheetz, Michael J Brownstein, Ted B Usdin, Shiraki Toshiyuki, Piero Carninci, Christa Prange, Sam S Raha, Naomi A Loquellano, Garrick J Peters, Rick D Abramson, Sara J Mullahy, Stephanie A Bosak, Paul J McEwan, Kevin J McKernan, Joel A Malek, Preethi H Gunaratne, Stephen Richards, Kim C Worley, Sarah Hale, Angela M Garcia, Laura J Gay, Stephen W Hulyk, Debbie K Villalon, Donna M Muzny, Erica J Sodergren, Xiuhua Lu, Richard A Gibbs, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha Madan, Stephanie Rodrigues, Amy Sanchez, Michelle Whiting, Anup Madan, Alice C Young, Yuriy Shevchenko, Gerard G Bouffard, Robert W Blakesley, Jeffrey W Touchman, Eric D Green, Mark C Dickson, Alex C Rodriguez, Jane Grimwood, Jeremy Schmutz, Richard M Myers, Yaron S N Butterfield, Martin I Krzywinski, Ursula Skalska, Duane E Smailus, Angelique Schnerch, Jacqueline E Schein, Steven J M Jones, Marco A Marra,

Proc. Natl. Acad. Sci. U.S.A., 2002 Dec 24 , 99, 16899-903

The National Institutes of Health Mammalian Gene Collection (MGC) Program is a multiinstitutional effort to identify and sequence a cDNA clone containing a complete ORF for each human and mouse gene. ESTs were generated from libraries enriched for full-length cDNAs and analyzed to identify candidate full-ORF clones, which then were sequenced to high accuracy. The MGC has currently sequenced and verified the full ORF for a nonredundant set of >9,000 human and >6,000 mouse genes. Candidate full-ORF clones for an additional 7,800 human and 3,500 mouse genes also have been identified. All MGC sequences and clones are available without restriction through public databases and clone distribution networks (see http:mgc.nci.nih.gov).

http://www.ncbi.nlm.nih.gov/pubmed/12477932