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Kir1.1

potassium inwardly-rectifying channel, subfamily J, member 1
Synonyms: Kir1.1 ROMK Romk2 Kcnj1. Symbol: Kcnj1

Introductions


The protein encoded by the gene KCNJ1 (also known as ROMK; ROMK1; KIR1.1) in humans is the integral membrane protein and inward-rectifier type potassium channel Kir1.1, which is member 1 of subfamily J. It is activated by internal ATP and probably plays an important role in potassium homeostasis. The encoded protein has a greater tendency to allow potassium to flow into a cell rather than out of a cell. Mutations in this gene have been associated with antenatal Bartter syndrome, which is characterized by salt wasting, hypokalemic alkalosis, hypercalciuria, and low blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene. http://www.ncbi.nlm.nih.gov/gene/3758

Inwardly rectifying potassium (Kir) channels are a class of potassium channels that, at a comparable driving force, allow greater influx than efflux of potassium ions. Their high open probability at negative transmembrane voltages makes them uniquely suited to set the resting membrane potential and control cell excitability. Unlike the intrinsic gating of voltage-dependent potassium channels, inward rectification is caused by voltage-dependent block of the channel pore by cytoplasmic ions, including Mg2+ and polyamines, such as spermine and putrescine [1853]

Genes


Kcnj1 : potassium inwardly-rectifying channel, subfamily J, member 1

RGD ID Chromosome Position Species
2957 8 32158243-32162370 Rat
736363 9 32180003-32206782 Mouse
732016 11 128707915-128737268 Human

Transcripts


Acc No Sequence Length Source
NM_017023 NCBI
NM_019659 NCBI
NM_001168354 NCBI
NM_153764 NCBI
NM_153766 NCBI
NM_153765 NCBI
NM_153767 NCBI
NM_000220 NCBI

Ontologies


Accession Name Definition Evidence

Interactions


Co expression with Kir7.1

In a high-throughput screen for small-molecule modulators of ROMK, we previously identified a potent and moderately selective ROMK antagonist, 7,13-bis(4-nitrobenzyl)-1,4,10-trioxa-7,13-diazacyclopentadecane (VU590), that also inhibits Kir7.1. Because ROMK and Kir7.1 are coexpressed in the nephron, VU590 is not a good probe of ROMK function in the kidney. Here we describe the development of the structurally related inhibitor 2,2'-oxybis(methylene)bis(5-nitro-1H-benzo[d]imidazole) (VU591), which is as potent as VU590 but is selective for ROMK over Kir7.1 and more than 65 other potential off-targets [1860]

Ph

The inward rectifier Kir1.1 (ROMK) family is gated by both internal pH and external K. Sackin [178]

Calcium and Magnesium

Kir1.1 inactivation, associated with transient internal acidification, is strongly dependent on external K, Ca, and Mg. Here, we show that in 1 mM K, a 15 min internal acidification (pH 6.3) followed by a 30 min recovery (pH 8.0) produced 84 ± 3% inactivation in 2 mM Ca but only 18 ± 4% inactivation in the absence of external Ca and Mg. In 100 mM external K, the same acidification protocol produced 29 ± 4% inactivation in 10 mM external Ca but no inactivation when extracellular Ca was reduced below 2 mM (with 0 Mg) [1858]

Ifenprodil

Kir1.1 and Kir2.1 channels are insensitive to ifenprodil [179]

E132, F127, and R128

Three residues (E132, F127, and R128) at the outer mouth of Kir1.1b directly affected inward rectifier gating by external K, independent of pH gating. Each of the individual mutations E132Q, F127V, F127D, and R128Y changed the normal K dependence of macroscopic conductance from hyperbolic (Km = 6 ± 2 mM) to linear, up to 500 mM, without changing the hyperbolic K dependence of single-channel conductance. This suggests that E132, F127, and R128 are responsible for maximal Kir1.1b activation by external K. In addition, these same residues were also essential for recovery of Kir1.1b activity after complete removal of external K by 18-Crown-6 polyether [1857]

Phosphatidylinositol 3,4-bisphosphate

Phosphatidylinositol 3,4-bisphosphate has an inhibitory effect on Kir1.1. [180]

Kir channels are only active when associated with phosphatidylinositol 4,5-bisphosphate (PIP) and therefore their activity is intimately coupled to the complex cellular metabolism of PIPs. Baukrowitz [904], Kobrinski [905], Xie [906]. The physiological importance of this phosphoinositide regulation is underlined by the observation that mutations in Kir1.1 channel which impair PIP2 interactions can lead to disease states such as the Bartter’s syndrome. Lopes [907]

Oleoyl-CoA,

ong chain fatty acid esters of coenzyme A (LC-CoA), e.g. oleoyl-CoA, potently and reversibly inhibits Kir1.1. [180]

K+

Also, it has been shown that changes in extracellular K concentrations can affect the sensitivity of ROMK channels to cell pH [1843]

Kir1 Inhibitors

The only inhibitors of Kir1.1 disclosed to date are variants of the bee venom peptide tertiapin (TPN) and two small molecules, VU590 and VU591, identified in a screening campaign at Vanderbilt University [1853]

Proteins


Structures


Structural model of an inward rectifier potassium channel

Kv.11.1 A homology model of the Kir1.1 channel cytoplasmic domain has been docked to the membrane-spanning portion of a Kir3.1–KirBac1.3 channel chimera. Regions of the channel backbone important for channel function, rectification and small-molecule binding are highlighted [1846]

GL: Gating loop; HBC: Helix bundle crossing; RC: Rectification controller; RM: Rectification modulator; SF: Selectivity filter; TM1 and 2: Transmembrane domains 1 and 2 The inward rectifier Kir1.1 (ROMK) family is gated by both internal pH and external K, where the putative pH gate is formed by the convergence of leucine side chains, near the inner helical bundle crossing at L160-Kir1.1. Structural disruption of the Kir1.1 bundle-crossing pH gate prevents both inactivation by low external K and reactivation by high external K. [178]

Distributions


SubCellular Distribution of Rat Kir1.1 (ROMK1) in Neuron

Kv.11.1 The channel is clearly targeted to the membrane, and somatic staining is very bright. Dendritic staining is also present. This pattern agrees with that seen with antibody staining (not shown). Because each channel is associated with one GFP molecule, relationship of functional channel to fluorescence is quantitative [1855]

Expressions


Kir1.1 Expressed in Rat Brain

The Kir1.1 channel has been seen to be expressed in several parts of the adult rat CNS. This includes the Olfactory Bulb, Granular Layer and Piriform Cortex [983]

Kir1 Predominantly Expressed in Kidney

Kir1.1, the product of the KCNJ1 gene, also known as renal outer medullary potassium (ROMK) channel, is unique in that its expression appears to be highly restricted to the kidney. Specifically, Kir1.1 channels are located at the apical membrane of epithelial cells lining the thick ascending loop of Henle (TALH) and the cortical collecting duct (CCD)13 and represent the molecular correlate of the small-conductance secretory potassium channel [1853]

Kir1.1 Expressed in Distal Convoluted Tubule

ROMK expression along the entire distal nephron, localizing to distal convoluted tubule regions, DCT1 and DCT2; the connecting tubule (CNT); and cortical collecting duct (CD). ROMK is diffusely distributed in intracellular compartments and at the apical membrane of each tubular region. Apical labeling was significantly increased by high-K diet in DCT2, CNT1, CNT2, and CD (P < 0.05) but not in DCT1. High-K diet causes a large increase in apical expression of ROMK in DCT2, CNT, and CD but not in DCT1, indicating that different regulatory mechanisms are involved in K diet-regulated ROMK channel functions in the distal nephron. Wade [908]

Functionals


Potassium Recycling

The ROMK channel plays an important role in K recycling in the thick ascending limb (TAL) and K secretion in the connecting tubule (CT) and cortical collecting duct (CCD). It is generally agreed that ROMK channels are mainly responsible for K secretion under normal conditions. [1843]

At the TALH (thick ascending loop of Henle), Kir1.1 participates in potassium recycling across the luminal membrane that is critical for the function of the furosemide-sensitive Na+/K+/2Cl− co-transporter, the rate-determining step for salt reuptake in this part of the nephron. At the CCD, Kir1.1 provides a pathway for potassium secretion that is tightly coupled to sodium uptake through the amiloride-sensitive epithelial sodium channel. Several dozen mutations in the KCNJ1 gene that cause loss of channel function have been identified in individuals with Bartter's syndrome type II, an autosomal recessive life-threatening disorder [1853]

PIP Metabolism

Kir channels are only active when associated with phosphatidylinositol 4,5-bisphosphate (PIP) and therefore their activity is intimately coupled to the complex cellular metabolism of PIPs. Baukrowitz [904], Kobrinski [905], Xie [906]. The physiological importance of this phosphoinositide regulation is underlined by the observation that mutations in Kir1.1 channel which impair PIP2 interactions can lead to disease states such as the Bartter’s syndrome. Lopes [907]

Disease and Pharmacology

Inward rectifier potassium (Kir) channels have been postulated as therapeutic targets for several common disorders including hypertension, cardiac arrhythmias and pain. With few exceptions, however, the small-molecule pharmacology of this family is limited to nonselective cardiovascular and neurologic drugs with off-target activity toward inward rectifiers. Consequently, the actual therapeutic potential and 'drugability' of most Kir channels has not yet been determined experimentally. The purpose of this review is to provide a comprehensive summary of publicly disclosed Kir channel small-molecule modulators and highlight recent targeted drug-discovery efforts toward Kir1.1 and Kir2.1 [1846]

Hypertension

Human and rodent genetic studies provide a large body of evidence which suggests that inhibitors of the kidney potassium channel, ROMK, will represent novel diuretics for the treatment of hypertension. The search for potent and selective ROMK inhibitors has recently yielded compounds that display efficacy in animal models, providing the first pharmacological validation of ROMK as a novel diuretic target.

Kinetics


Single Channel Conductance

Patch-clamp experiments demonstrated that two types of K channels, a 30- to 40- and a 70- to 80-pS, are expressed in the apical membrane of the TAL (thick ascending limb ). It is well established that the 30-pS K channel is related to ROMK because it has similar biophysical properties and regulatory mechanisms to that in native tubules. However, it is not clear whether ROMK is also involved in forming the apical 70-pS K channel. [1843]

Single Channel Conductance of Kir1.1 in Apical Membrane of Mouse Cortical TAL

Kir1.1 Single–channel I-V curve (A, upper right) shows slight inward rectification with a slope conductance of 32.1 pS [1854]

Rat ROMK1 Expressed in HEK293 Cells

Kir1.1 A: voltage step protocol, with test potentials between −140 and +60 mV (holding at −80 mV) for the detection of functional ROMK1. B: HEK 293 cell transfected with ROMK1-CITE-EGFP. C: the same cell with constant perfusion of 500 μM Ba2+. D: Ba2+ washout. E and F: a mock-transfected cell with and without Ba2+ showing some nonspecific leak currents [1855]


Kinetics

The kinetics of the Kir family do not follow the Hodgkin Huxley Kinetics model. This is because its kinetics depends not only on the membrane potential but also on the equilibrium potential for K+. The channel conductance increases as the concentration of extra cellular K+ increases [1844]

Kir1 Kinetics in A gambiae Mosquitos

when bathed in a control (ND96) solution, heterologous expression of AgKir1 channels in Xenopus oocytes gave rise to functional channels exhibiting spontaneous large inward and small outward currents between −140 mV and −80 mV. Moreover, the spontaneous resting membrane potential of the AgKir1-expressing oocytes (−96.9 ± 1.3 mV) was (1) hyperpolarized compared to the H2O-injected oocytes (−42.0 ± 2.5 mV), and (2) close to the estimated Nernst potential for K+ (−102 mV). The inward AgKir1 channel currents increase with increased extracellular K+ concentrations and are inhibited by barium. These functional characteristics are canonical of most animal Kir channels, and very similar to those of AeKir1. Thus, AgKir1 functions as an inward rectifier K+ channel [1852]

Human Kir1.1 Expressed in CHO Cells

Human Kir1.1, stably expressed in CHO cells, produced large, stable currents on the IonWorks HT platform. Using a 0.1-s ramp from −100 to +100 mV as the voltage command, the recorded currents displayed inward rectifying properties and were inhibited by the addition of 5 mM BaCl2. Incubation with 5 mM BaCl2 is expected to completely block currents conducted by Kir1.1 channels, and the remaining current is presumed to be a nonspecific “leak” current, resulting from the low resistance seal between the cell and the plastic substrate used to manufacture the disposable PatchPlate™. To minimize the contribution of leak currents to currents recorded from hKir1.1-expressing cells, the preferred protocol (protocol 2) combined near-physiological extracellular potassium concentrations (10 mM) with a test pulse voltage of 0 mV [1853]

Models


Hodgkin and Huxley Model Prediction for Kir1.1

Kir1.1 This shift, which quantifies silencing as a function of ROMK1 expression, was predicted from Hodgkin-Huxley models [1851]

References


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[1843 : 16339961]
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Credits